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                                                                                                                                微生物檢測的技術方法
                                                                                                                                來源:后王電子 編輯:深圳后王發布日期:2016-08-17 15:35:05

                                                                                                                                食品安全檢測箱

                                                                                                                                深圳后王電子所了解MPN (most probable number)法即最近似數法,也稱為最大可能數法,是食品檢驗中常用的方法.其生化反應基礎為乳糖發酵,革蘭陰性菌的無芽胞桿菌,如大腸菌群,一般在 37℃、24h 發酵乳糖、產酸產氣.利用此原理,將供試液加到發酵培養基中,37℃培養24h.若不產氣,則報告控制菌陰性;若產氣,則需劃線接種于平板上,37℃培養24h 后,觀察菌落形態、革蘭染色和重發酵驗證.

                                                                                                                                優點:操作簡便、靈敏度較高,時間短,可直接觀察試管得出結論,若發酵培養基不產氣,24h 即可報告控制菌陰性.

                                                                                                                                缺點:要求樣品具有均一性,適合檢驗液體樣品,且只能用于檢驗在發酵培養基中產氣的菌種.注意事項:需選擇合適的稀釋度和接種量.

                                                                                                                                CFU(colony forming units)法即平板計數法,是食品和藥品檢驗中常用的方法.原理為lg 或lml 樣品中所含的活菌數量,通過將處理后的樣品在一定條件下培養后計算生長出來的菌落數(CFU),即在一定體積的細菌培養液在固態培養基上形成的菌落數.液體樣品可直接稀釋成供試液,固體樣品需在滅菌的生理鹽水中浸泡制成供試液.將不同稀釋度的供試液吸取lml,放入滅菌的平皿內,再傾注融化的營養瓊脂,待瓊脂凝固后,翻轉平皿,37℃培養4d,計算平皿內的菌落數.CFU法應用廣泛,但檢驗過程必須嚴格無菌,以防污染雜菌,而且檢驗所需時間較長,同時也需選擇合適的稀釋度和接種量.

                                                                                                                                深圳后王電子科技有限公司所生產的微生物食品安全檢測箱采用的CFU法能檢測大腸桿菌、大腸菌群、蠟樣芽孢桿菌、金黃色葡萄球菌、阪崎腸桿菌、副溶血弧菌、霉菌、酵母菌、沙門氏菌等項目。


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